Data to Be Collected

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Data to Be Collected
Experiment 1:
The average density of honey bees ([beginning ending] / 2) for each cage was determined to be 245, 1,008, 4,186 (for the 400,1,600, 6,400 bee groups, respectively) for the year. The number of unopened florets per raceme was determined for tagged racemes on the Climax plants in each plot during March before inserting bee colonies into cages at stage five of blooming (Spiers 1978). To compensate for wind loss of tags, we marked double as many racemes in the open plots. On several days during the bloom period (early March through early April), will measure for each plot the rate of legitimate honey bee flower visits (number of legitimate flower visits per min) during normal flight hours. Harvest of fruits started at the beginning of June until the middle of July in every year. The following dependent variables were measured for each recovered raceme: percent fruit set, speed of ripening, Green fruit set, fruit set/cluster%, Ripe fruit set, Maturation, berry weight (g), Soluble solids, Immature seeds, Mature seeds, Total seed set, number of seeds per fruit.
“Fruit set” is the proportion of flowers that have successful pollination and a berry develops. Percent fruit set = (berry number/flower number) x 100 was determined for each raceme with ripe fruit in year 1 or full-sized green fruit in year 3. Number of seeds per fruit was determined by expressing berry contents and counting the number of fully formed seeds. Speed of ripening was calculated for only 1 yr as the percentage of fruits ripe on one arbitrarily-chosen date. “Fruit bud set”: The proportion of buds on a lateral shoot that are flower (fruit) buds. Flower bud differentiation starts at the base of the cluster, count Number of flowers per bud. Flower bud initiation” (FBI): When a vegetative bud changes to a floral bud. “Flower bud differentiation” (FBD): When flowers and floral parts develop within the bud number of berries develop per flower bud or per inflorescence. yield/plant, Total yield per bush, cumulative yield, mean berry weight and mean berry dry weight

Experiment 2:
Green fruit set, Berries from bagged terminals (−x-pollination) and unbagged ones ( x-pollination) were harvested on 7 June, 9 June, 22 June, 30 June, and 1 July and analyzed for yield (kg), wet weight (g), soluble solids content (%), and seed set with two-way ANOVA and Tukey’s HSD tests (PROC GLM, SAS Institute, 2001). During the research period, bee activity in the block was monitored carefully. Gibberellic acid was applied to three to five individual flower clusters of plants (30 flowers per plant) when the flowers were at the desired stage of development. Bud development was monitored based on the stage descriptions of Spiers (1978): 5 = individual flowers distinctly separated, corollas unexpanded; 6 = corollas completely expanded; Before spray GA3 for the experiment 2, flower clusters were tagged and the number of flowers per cluster was recorded. Single applications, need to measure each flower bud stage, for each stage need also to include the percentage of Fruit set/cluster. Fruit set was determined by counting fruit 4 to 6 weeks after petal fall to insure that the major period of fruit abortion was past (Davies, 1986). After a substantial number of berries had ripened on all treatments, mature fruit were harvested from flower clusters to determine their fresh weight and soluble solids concentration (SSC). Fruit set percentage data were subjected to arcsine transformation before analyses, and Green fruit set, fruit set/cluster%, Ripe fruit set, Maturation, Seed number per fruit, Fruit weight, Soluble solids, Immature seeds, Mature seeds, Total seed set were analyzed using appropriate analysis of variance and regression procedures. yield/plant.
Berries from bagged terminals (−x-pollination) and unbagged ones ( x-pollination) were harvested on 10 June, 17 June, 24 June, 2 July and 9 July and analyzed for yield (kg), wet weight (g), soluble solids content (%), and seed set with two-way ANOVA and Tukey’s HSD tests (PROC GLM, SAS Institute, 2001).

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